3D Variability Analysis (3DVA) is a computational method to resolve continuous and discrete heterogeneity from single particle cryo-EM data of protein molecules. 3DVA is implemented in cryoSPARC and has been available to use since version v2.9 (June 2019).
Below are videos of the flexible continuous motion that is discovered by 3DVA in a range of molecules. Please see the bioRxiv preprint for more details.
Movie S1. Results of 3DVA on Pf80S Ribosome particles, showing four variability components that capture: 1) missing head region 2) rotation of 40S subunut 3) lateral shift of 40S subunit 4) rotation of head region.
Movie S2. Results of 3DVA with three components on Cannabinoid GPCR complex. 1) bending of CB1 transmembrane domain towards G-protein. 2) transverse bending of CB1 and motion of G_beta-G_gamma helices. 3) twisting of CB1 transmembrane domain.
Movie S3. Results of 3DVA with three components on Nav1.7 Ion channel. 1) bending of transmembrane region, along with flex of Fabs together/apart. 2) bending of 4-helix bundle. 3) bending of peripheral transmembrane regions of two subunits not bound to Fabs.