= nucleoside analog → build in instead of thymidine
BrdU positive and negative, quantify does not really matter.
When give BrdU
→ dividing unlabeled cell will become 2 labeled cells. → gain 2 labeled cells.
→ from 1 already labelled cell to 2 labelled cells → gain 1 labelled cell.
After giving BrdU:
U+L=1 → always scale it so 1 → so you can use one equation to solve the other.
But a lot of toxicity
Deuterium 2H → how much deuterium goes into the T cells. = measure of turnover the cells.
Gather information during uplabeling and downlabeling bc you do it for longer period of times.
Expressing it at the level of DNA