https://s3-us-west-2.amazonaws.com/secure.notion-static.com/4fecdba0-208d-4bd0-8a0d-c707b085131d/Untitled.png

A What would your synthetic cell do? What is the input and what is the output.   we want make our synthetic cell to have a sensor to detect the PAH and PM2.5.

Do to the 3D printer have emission the Pm2.5 and PAHs(Polycyclic Aromatic Hydrocarbons ) which is bad for human. I hope to produce a biosensor that to detect the PM2.5 and PAH putting next to the 3D printer.In put: the sensor and receptor is to detect the PAHs and some PAHs.

Out put: Color change indicating which the biosensor can detect Particular matters (PM2.5) and PAHs.(What is PAHs)Polycyclic aromatic hydrocarbons

Polycyclic aromatic hydrocarbons(PAHs) are a large group of organic  compounds with two or more fused aromatic rings.  They have a relatively low  solubility in water, but are highly lipophilic. Most of the PAHs with low vapour pressure in the air are adsorbed on particles. When dissolved in water or adsorbed on particulate matter, PAHs can undergo photodecomposition when exposed to ultraviolet light from solar radiation**.**

https://lh3.googleusercontent.com/piVQCiV3wJDDB4O874hdGLL2337QU5h1CPk-rhK6iChy-e_wN06pDp7CxDfebWOj0vxVCjjVLMf1nNO7SdFSr65hMyYP_GLExFfhNiNVcL2tow9Y5MS5atl8K7PUNcao

B Could this function be realized by cell free Tx/Tl alone, without encapsulation?

No. I think it cannot. C Could this function be realized by genetically modified natural cell? yes, but I think it would limit the repertoire of possible odorants, as some of them might be toxic to the cell, or be antagonists for native receptors.

**D Describe the desired outcome of your synthetic cell operation.**The artificial cell, will have a set PAHs receptors, like the ones we have in our nose that would be activated by the mal-odorants and subsequently would trigger the release of nice smelling odorants, through a gated membrane channel.

Reference

  1. Zhang, Peng, et al. "Bringing light into cell-free expression." ACS Synthetic Biology 9.8 (2020): 2144-2153.
  2. Levskaya, Anselm, et al. "Engineering Escherichia coli to see light." Nature 438.7067 (2005): 441-442.
  3. Liu, Zedao, et al. "Programming bacteria with light—sensors and applications in synthetic biology." Frontiers in microbiology 9 (2018): 2692.
  4. Khalil, Ahmad S., and James J. Collins. "Synthetic biology: applications come of age." Nature Reviews Genetics 11.5 (2010): 367-379.
  5. Ramakrishnan, Prabha, and Jeffrey J. Tabor. "Repurposing synechocystis PCC6803 UirS–UirR as a UV-violet/green photoreversible transcriptional regulatory tool in E. coli." ACS synthetic biology 5.7 (2016): 733-740.