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1. Assemble and fill the potometer, submerging the capillary tube in a beaker of water
2. Cut a shoot underwater to prevent air entering the xylem (cut at a slant to increase surface area for water uptake) and insert the shoot underwater
3. Check the apparatus is watertight and airtight
4. Dry the leaves and shut the tap
5. Remove the end of the capillary tube from the beaker until a single air bubble has formed, then put it back
6. Record the starting position of the bubble, start a stopwatch and record the distance moved per hour
7. Repeat, calculate mean, exclude anomalies

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Control variables

• Keep temperature the same
• Keep air humidity the same
• Keep light intensity the same </aside>

You can estimate rate of transpiration (loss of water through the leaves) by measuring the uptake of water by the plant – the bubble acts as a visual representation for how much water is absorbed

MAKE SURE YOU RECOGNISE THE DIFFERENCE BETWEEN:
THIS POTOMETER EXPERIMENT  (FOR RATE OF TRANSPIRATION) 
AND THE LIGHT INTENSITY EXPERIMENT (FOR RATE OF PHOTOSYNTHESIS)

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Factors that affect rate of transpiration 🫧