Overview

This guide will introduce you to Nucleus Hub for accessing the Cell Development Kit (CDK) and collaborating with other Nucleus Developers.

Nucleus Hub Registration

Currently, access to Nucleus Hub is available by request to build@bnext.bio.

• [ ] Navigate to hub.nucleus.engineering.
• [ ] Follow the prompts to make an account.
• [ ] After clicking Create User , send an email to build@bnext.bio to approve the account.

Orientation

Upon entering Nucleus Hub you should the following screen.

1. File directory.
2. Launcher window.
3. Make a new empty Jupyter Notebook.
4. Make a new template Jupyter Notebook.
5. Preview a Developer Note.
6. Open a terminal window.
7. View a draft of a Developer Note.

Accessing the Cell Development Kit

Nucleus Hub makes it easy to access the CDK to design and analyze experiments. This section will demonstrate a simple workflow for analyzing fluorescence time series data obtained from a microplate reader.

• [ ] Open the /sample-data/ directory.
• [ ] Click the Template icon, select /analysis/platereader.ipynb, and click GO.
• [ ] Run the first cell to import the platereader library from the CDK.