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The IV-HSL Emitter module is a set of two genetic constructs. The first produces a type of homoserine lactone called IV-HSL through an expressed enzyme pathway. The second activates expression in response to IV-HSL, enabling amplification of the signal.
The IV-HSL production module is a genetic construct pT7-bjaI that encodes the enzyme BjaI. This enzyme converts S-adenosylmethionine (SAM) and isovaleryl coenzyme A (IV-CoA) into N-isovaleryl-l-homoserine lactone (IV-HSL). IV-HSL is a membrane-permeable acyl homoserine lactone.
The IV-HSL receiver module is an E. coli genetic construct bjaR-GFP-native that encodes the transcription factor bjaR that promotes downstream gene expression in response to picomolar concentrations of IV-HSL [Lindemann, 2011].
pT7-bjaI is MoClo compatible as a Level 0 CDS part, and may be assembled into a Level 1 transcription unit using BsaI.
<aside> <img src="/icons/chemistry_gray.svg" alt="/icons/chemistry_gray.svg" width="40px" /> Usage
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The IV-HSL emitter module may be implemented by assembling the pOpen-pT7-bjaI DNA construct into a standard PURE reaction, following Assemble PURE Reactions. Add equimolar amounts of the substrates SAM and IV-CoA at 0.3 uM and 0.08 uM final concentration, respectively.
DNA Parts
pT7-bjaI—Nucleus v0.3.0 Distribution Plate upcoming.bjaR-GFP-native—Nucleus v0.3.0 Distribution Plate upcoming.Protein Components
Cell Components
Reaction Construction