<aside> 📎 Red: Unsolved Orange: Being Processed Green: Solved

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Objectives of the Experiment

  1. To create two simulated cell expression matrices with known cell type proportions
  2. To apply methods found in the literature to find the signature gene markers of the cell types in the simulated cell expression matrix
  3. To apply methods found in the literature to determine with statements of statistical significance the proportions of cells in respective cell matrices

Procedures

1. Generation of Cell Expression Matrices

1. Generation of the First Cell Expression Matrix (Exp1.1.R)

2. Adapting countdc to Seurat pipeline and generate clusters (Exp 1.2.R)

<aside> ⚠️ The workflows in the tutorial of Seurat vignette are adapted for this step: https://satijalab.org/seurat/v3.1/pbmc3k_tutorial.html

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