Arenas define the space in which flies can move. There are several types of behavioural arenas that can be used with the ethoscope. Most users will work with the regular arena, which was initiatially provided in the ZIP bundle (https://zenodo.org/record/831153) associated with our paper.
<aside> ☝ Very important. Arenas must have "targets". Targets are 4mm-diameter black disks that figure at three of the four corners of the arena, at constant positions. Targets are essential for the software to define regions of interest. You will not be able to start tracking experiments without targets! A regular printer, a pair of scissors and some paper glue will do the job. The ZIP bundle also contains targets on an A4 page that you can print. You need to ensure the target are circular, contracted and visible.
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All our arenas are available on onshape. A PDF with printable targets is found below.