We are going to run a subset of our indexed primer sets (RPP3, S2, N1) with NTC reactions to diagnose molecular contamination with our DNA spike.
Samples to Run:
1 row of 20X N1 primer mix
1 row of 20x S2 primer mix
1 row of 20x RPP primer mix (N1)
1 row of 20x RPP primer mix (S2)
1 row of 20x N1/RPP primer mix
1 row of 20x S2/RPP primer mix
Individual Reaction for singleplex Mixes
10 mcL Luna Mix
1 mcL RT enzyme mix
1 mcL primer mix
8 mcL H20
Individual Reaction for duplex Mixes
10 mcL Luna Mix
1 mcL RT enzyme mix
2 mcL primer mix