We are going to run a subset of our indexed primer sets (RPP3, S2, N1) with NTC reactions to diagnose molecular contamination with our DNA spike.

Samples to Run:

1 row of 20X N1 primer mix

1 row of 20x S2 primer mix

1 row of 20x RPP primer mix (N1)

1 row of 20x RPP primer mix (S2)

1 row of 20x N1/RPP primer mix

1 row of 20x S2/RPP primer mix

Individual Reaction for singleplex Mixes

10 mcL Luna Mix

1 mcL RT enzyme mix

1 mcL primer mix

8 mcL H20

Individual Reaction for duplex Mixes

10 mcL Luna Mix

1 mcL RT enzyme mix

2 mcL primer mix